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KMID : 0364819760140020049
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Korean Journal of Microbiology
1976 Volume.14 No. 2 p.49 ~ p.56
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Studies on the Enzyme Activities of Rhizopus distributed in South Korea (I); On the Amylase, protease and cellulase activities
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이영녹
윤경하/이평우/배광성/박용근/정성균/서항원
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Abstract
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Enzyme activities, such as glucoamylase dextrinogenic amylase, cellulase, acid protease and neutral protease, of Rhizopus isolated from various substrates collected throughout South Korea are measured, and their enzyme activities are surveyed from taxonomical, ecological and physiological viewpoint. Effect of carbon sources and phytohormones on the amylase production of Rhizopus are also measured. Among the 735 strains of Rhizopus isolated, strain number 587 exhibiting most prominent dextrinogenic amylase and neutral protease activity is selected as the best strain, and the strain number 673, 108, 329, 165 and 728 are selected for their predominant cellulase, acid protease, glucoamylase, dextrinogenic amylase and neutral protease activities, respectively. R.acidus and R.nigricans which exhibited relatively higher callulalse activity, showed lower activities for both amylase. R.tritici exhibited higher protease activity. The relations between activities and various substrates of wild strains are not outstnading difference, although the strains isolated from inland region exhibited more or less higher amylase and cellulase activities, than those of coast region, generally. Lactose and dextrin are most effective carbon sources for glucoamylase and dextrinogenic amylase production of the Rhizopus niveus, respectively. Although all phytohormones tested are effective for production of amylase by the Rhizopus strains, except nicotinamide for glucoamylase production, biotin and ascorbate are most effective for dextrinogenic amylase and glucoamylase production, respectively.
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KEYWORD
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